Journal: Antioxidants

Article Title: 5-HEPE Ameliorates Aging of Duck Ovarian Granulosa Cells by Targeting FOXM1 and Suppressing Oxidative Stress

doi: 10.3390/antiox14121425

Figure Lengend Snippet: 5-HEPE alleviated oxidative stress and upregulated FOXM1 expression in KGN cells. ( A ) Differential omics profile in plasma and ovary. ( B ) Cell viability of KGN cells after treatment with different concentrations of H 2 O 2 . ( C – E ) GSH, SOD, and MDA content in KGN cells. ( F , G ) Gene and protein expression of GPX4 in KGN cells. ( H , I ) Gene and protein expression of FOXM1 in KGN cells. ( n = 3). Data are presented as the mean ± SEM, with statistical significance denoted as * p < 0.05, ** p < 0.01.

Article Snippet: The immortalized human granulosa cell line KGN cells were acquired from Procell (Wuhan, China) and cultured in DMEM/F12 medium containing 10% fetal bovine serum and 1% penicillin–streptomycin.

Techniques: Expressing, Clinical Proteomics